Integration of FRET and sequencing to engineer kinase biosensors from mammalian cell libraries

Longwei Liu
Praopim Limsakul
Xianhui Meng
Yan Huang
Reed E. S. Harrison
Tse-Shun Huang
Yiwen Shi
Yiyan Yu
Krit Charupanit
Sheng Zhong
Shaoying Lu
Jin Zhang
Shu Chien
Jie Sun
Yingxiao Wang

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Publication date

August 2021

DOI

10.1038/s41467-021-25323-x

Publisher

Springer Science and Business Media LLC

Journal

Nature Communications

Abstract

Existing Förster Resonance Energy Transfer (FRET) biosensors are often limited in their sensitivity. Here the authors report FRET-seq which they use to identify Fyn and ZAP70 kinase biosensors with enhanced performance, and use them to image T-cell activation and screen drugs

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Integration of FRET and sequencing to engineer kinase biosensors from mammalian cell libraries

Abstract

Extracted data

Integration of FRET and sequencing to engineer kinase biosensors from mammalian cell libraries

Abstract

Extracted data

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