Although symmetric peaks with Gaussian shapes are predicted by models of the chromatographic process, "perfect peaks" are not observed often outside of textbooks. Several physico-chemical phenomena can lead to asymmetric peak shapes, including analyte adsorption to different types of sites within the stationary phase, and overload tailing, which may involve a variety of factors. Understanding these phenomena can help identify whether the cause of asymmetry is most likely to have a physical or chemical origin, which in turn dictates which troubleshooting steps to start with when dealing with poor peak shapes
© 2015 Elsevier B.V. Some factors which present difficulties for obtaining good peak shape in hydrop...
The efficiency of chromatographic separations decreases markedly when peaks exhibit asymmetry (e.g.,...
Errors in an analytical method are often not recognized as such or are underrated. Therefore, it is ...
Although symmetric peaks with Gaussian shapes are predicted by models of the chromatographic process...
While other separation mechanisms can challenge the dominance of reversed phase (for example in the ...
Three graphs that indicate the relative error in peak area when two incompletely resolved peaks are ...
Various aspects of chromatographic peak quantitation and shape characterization are investigated in ...
Automatic peak evaluation in chromatograms and subsequent quantification of compound concentrations ...
Due to the character of the original source materials and the nature of batch digitization, quality ...
The elution profile of chromatographic peaks provides fundamental understanding of the processes tha...
Quantitative chromatographic analysis is liable to errors due to peak asymmetry because the uncertai...
Chromatograms with overlapping peaks and a baseline rise or upset constitute a great challenge for a...
In chromatographic processes, molecular collisions between the mobile phase and the solute result ...
Abstract: Mathematical methods for separation of overlapping asymmetrical peaks in spectroscopy and ...
Peak profiles in chromatography are characterised by their height, position, width and asymmetry; th...
© 2015 Elsevier B.V. Some factors which present difficulties for obtaining good peak shape in hydrop...
The efficiency of chromatographic separations decreases markedly when peaks exhibit asymmetry (e.g.,...
Errors in an analytical method are often not recognized as such or are underrated. Therefore, it is ...
Although symmetric peaks with Gaussian shapes are predicted by models of the chromatographic process...
While other separation mechanisms can challenge the dominance of reversed phase (for example in the ...
Three graphs that indicate the relative error in peak area when two incompletely resolved peaks are ...
Various aspects of chromatographic peak quantitation and shape characterization are investigated in ...
Automatic peak evaluation in chromatograms and subsequent quantification of compound concentrations ...
Due to the character of the original source materials and the nature of batch digitization, quality ...
The elution profile of chromatographic peaks provides fundamental understanding of the processes tha...
Quantitative chromatographic analysis is liable to errors due to peak asymmetry because the uncertai...
Chromatograms with overlapping peaks and a baseline rise or upset constitute a great challenge for a...
In chromatographic processes, molecular collisions between the mobile phase and the solute result ...
Abstract: Mathematical methods for separation of overlapping asymmetrical peaks in spectroscopy and ...
Peak profiles in chromatography are characterised by their height, position, width and asymmetry; th...
© 2015 Elsevier B.V. Some factors which present difficulties for obtaining good peak shape in hydrop...
The efficiency of chromatographic separations decreases markedly when peaks exhibit asymmetry (e.g.,...
Errors in an analytical method are often not recognized as such or are underrated. Therefore, it is ...