Three stop codons (UAA, UAG and UGA) terminate protein synthesis and are almost exclusively recognized by release factors. Here, we design de novo transfer RNAs (tRNAs) that efficiently decode UGA stop codons in Escherichia coli. The tRNA designs harness various functionally conserved aspects of sense-codon decoding tRNAs. Optimization within the T Psi C-stem to stabilize binding to the elongation factor, displays the most potent effect in enhancing suppression activity. We determine the structure of the ribosome in a complex with the designed tRNA bound to a UGA stop codon in the A site at 2.9 angstrom resolution. In the context of the suppressor tRNA, the conformation of the UGA codon resembles that of a sense-codon rather than when canon...
In contrast to bacteria that have two release factors, RF1 and RF2, eukaryotes only possess one unre...
We report the first systematic evolution and study of tRNA variants that are able to read a set of U...
A series of site-directed mutations has been constructed in E coli 16S rRNA and shown to suppress UG...
Here, the authors report de novo design, optimization and characterization of tRNAs that decode UGA ...
Pseudouridylation of messenger RNA emerges as an abundant modification involved in gene expression r...
Translation termination in bacteria involves precise reading of stop codons (UAA, UAG, UGA) and coor...
The deletion of the highly conserved cytidine nucleotide at position 1054 in E. coli 16S rRNA has be...
Termination of protein synthesis is triggered by the recognition of a stop codon at the ribosomal A ...
Understanding translation efficiency is crucial to pharmaceutical companies that have invested subst...
When the A site of the ribosome encounters a stop codon, a cytoplasmic protein release factor (RF) b...
Involvement of E. coli 23S ribosomal RNA (rRNA) in decoding of termination codons was first indicate...
Involvement of E. coli 23S ribosomal RNA (rRNA) in decoding of termination codons was first indicate...
The decoding release factor (RF) triggers termination of protein synthesis by functionally mimicking...
The structures of the ribosome and its subunits are now available at atomic detail, as well as those...
Accurate translation termination by release factors (RFs) is critical for the integrity of cellular ...
In contrast to bacteria that have two release factors, RF1 and RF2, eukaryotes only possess one unre...
We report the first systematic evolution and study of tRNA variants that are able to read a set of U...
A series of site-directed mutations has been constructed in E coli 16S rRNA and shown to suppress UG...
Here, the authors report de novo design, optimization and characterization of tRNAs that decode UGA ...
Pseudouridylation of messenger RNA emerges as an abundant modification involved in gene expression r...
Translation termination in bacteria involves precise reading of stop codons (UAA, UAG, UGA) and coor...
The deletion of the highly conserved cytidine nucleotide at position 1054 in E. coli 16S rRNA has be...
Termination of protein synthesis is triggered by the recognition of a stop codon at the ribosomal A ...
Understanding translation efficiency is crucial to pharmaceutical companies that have invested subst...
When the A site of the ribosome encounters a stop codon, a cytoplasmic protein release factor (RF) b...
Involvement of E. coli 23S ribosomal RNA (rRNA) in decoding of termination codons was first indicate...
Involvement of E. coli 23S ribosomal RNA (rRNA) in decoding of termination codons was first indicate...
The decoding release factor (RF) triggers termination of protein synthesis by functionally mimicking...
The structures of the ribosome and its subunits are now available at atomic detail, as well as those...
Accurate translation termination by release factors (RFs) is critical for the integrity of cellular ...
In contrast to bacteria that have two release factors, RF1 and RF2, eukaryotes only possess one unre...
We report the first systematic evolution and study of tRNA variants that are able to read a set of U...
A series of site-directed mutations has been constructed in E coli 16S rRNA and shown to suppress UG...