We aim to better understand the heating of the gas by observing the prominent gas cooling line [CII] at 158um in the low-metallicity environment of the Local Group spiral galaxy M33 at scales of 280pc. In particular, we aim at describing the variation of the photoelectric heating efficiency with galactic environment. In this unbiased study, we used ISO/LWS [CII] observations along the major axis of M33, in combination with Herschel PACS and SPIRE continuum maps, IRAM 30m CO 2-1 and VLA HI data to study the variation of velocity integrated intensities. The ratio of [CII] emission over the far-infrared continuum is used as a proxy for the heating efficiency, and models of photon-dominated regions are used to study the local physical densities...