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Not AvailableBlack tiger shrimp Penaeus monodon from 19 locations of Andaman and Nicobar Islands were studied for genetic differentiation and variability using allozyme electrophoretic analysis. Thirty two presumed loci were consistently expressed from 25 enzymes and a general protein, and these were also temporaly stable during the period 2002-2005. The percentage of polymorphic loci, mean number of alleles, mean effective number of alleles, observed homozygosity, expected homozygosity, observed heterozygosity, expected heterozygosity, average heterozygosity, gene flow (Nm), Fis, Fit, Fst, Rare alleles, monomorphic loci, diagnostic loci, genetic identity, genetic distance and establishment of breeding boundaries were documented in all tested populations, and between the population of Andaman group and Nicobar group of islands. Colonization of P. monodon population due to the decrease of genetic diversity has been formed from north Andaman to south Andaman. As a result, two insular populations i.e., A (Andaman group of islands) and B (Nicobar group of islands) were differentiatedNot Availabl