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Not AvailableBottle gourd [Lagenaria siceraria (Molina) Standle] is one of the most widely grown cucurbit in India valued for its various medicinal and nutritional properties. The crop is plagued by a multitude of diseases which drastically affect the yield and quality of produce. Under South Indian conditions, Gummy Stem Blight (GSB) caused by Didymella bryoniae is creating havoc wiping out entire populations during recent times. Till now there are no reported sources of resistance to GSB. The present study is aimed at screening advanced breeding lines of bottle gourd for GSB along with powdery mildew, CGMMV and phyllody. We found that three lines BG 95-3, 95-4 and 95-6 were showing moderate to high resistance to GSB. Among these 95-3 and 95-4 were also showing high resistance to powdery mildew with the absence of CGMMV and phyllody infection. Twelve lines were found to have low to medium resistance to GSB however with excellent yield attributes. The lines identified can be successfully utilized to breed high yielding multi-disease resistant bottle gourd varieties.Not Availabl