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Not AvailableComputer vision with deep-learning is emerging as a major approach for non-invasive and non-destructive plant phenotyping. Spikes are the reproductive organs of wheat plants. Detection and counting of spikes considered the grain-bearing organ have great importance in the phenomics study of large sets of germplasms. In the present study, we developed an online platform “Web-SpikeSegNet” based on a deep-learning framework for spike detection and counting from the wheat plant’s visual images. The architecture of the Web-SpikeSegNet consists of 2 layers. First Layer, Client-Side Interface Layer, deals with end user’s requests and corresponding responses management. In contrast, the second layer, Server Side Application Layer, consists of a spike detection and counting module. The backbone of the spike detection module comprises of deep encoder-decoder network with hourglass for spike segmentation. The Spike counting module implements the “Analyze Particle” function of imageJ to count the number of spikes. For evaluating the performance of Web-SpikeSegNet, we acquired the wheat plant’s visual images, and the satisfactory segmentation performances were obtained as Type I error 0.00159, Type II error 0.0586, Accuracy 99.65%, Precision 99.59% and F1 score 99.65%. As spike detection and counting in wheat phenotyping are closely related to the yield, Web-SpikeSegNet is a significant step forward in the field of wheat phenotyping and will be very useful to the researchers and students working in the domain.Not Availabl