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Not AvailableThe current study investigated how Saccharomyces cerevisiae ameliorates the adverse effects of aflatoxin on in vitro rumen fermentation. In this study, five groups (T1: Control [basal feed]; T2: T1 + 300 ppb aflatoxin B1 [AFB1] and T3, T4, and T5: T2 with 0.05, 0.1, and 0.2% of S. cerevisiae, respectively) were prepared and incubated in vitro. The results revealed that truly degradable dry matter (TDDM), gas production (GP), microbial biomass production (MBP), truly degradable organic matter (TDOM), partitioning factor (PF), total volatile fatty acids (TVFA), acetate (A), propionate (P) and butyrate (B) values in the control group (T1) were higher (p < 0.05) than those of the AFB1 fed group (T2). The A : P ratio in the control group (T1) was reduced (p < 0.05) when compared to that of the T2 group. The TDDM, TDOM, GP, TVFA, A, P, and B values of T3, T4, and T5 improved with the increasing levels of S. cerevisiae; however, the values of group T5 were lower (p < 0.05) than that of the control. The values of MBP, A : P ratio and PF in group T5 were statistically similar to that of the control. It was concluded that the inclusion of S. cerevisiae (0.05 to 0.20%) to the AFB1 (300 ppb) contaminated feed partially to completely ameliorated the adverse effects of AFB1 on the in vitro rumen fermentation parameters.National Research Foundation of South Kore