Not Available

Not AvailablePolyploidization plays an important role in the genesis of cultivated wheat (hexaploid and tetraploid) fromits diploid progenitors. Thus, evolution during polyploidization resulted in present-day hexaploid bread wheat. GS2 and Fd-GOGAT enzymes are core components involved in the assimilation of primary nitrogen (N) in plants. In the present study, we aimed to analyze these two important genes at their molecular level to find the extent of variation that occurred during evolution from the ancient diploid progenitors to the modern-day hexaploid bread wheat. Furthermore, we studied their gene expression pattern and assayed both the enzymes under N stress. We also investigated the degree of resilience among these species in terms of certain important morphophysiological and biochemical parameters under N stress. Comparison of the genomic sequences along with their promoter region revealed that both GS2 and Fd-GOGAT genes were located on chromosome 2 and were comprised of 13 and 33 exons respectively. A limited sequence divergence at cDNA and amino acid levels was observed among the genome species, but the divergence was significantly higher in the promoter region. Both these genes were present in three copies in the bread wheat, two copies in the durum wheat, and a single copy in their diploid progenitors. Differential gene expression among the five genome species under N stress suggested major differences in gene regulation due to the difference in cis-elements. Enzyme activity did not correlatewith the gene expression level probably due to post-transcriptional and post-translationalmodification of the enzymes. There was neither correlation between the GS2 and Fd-GOGAT activity in any species. All growth parameters, except root length, decreased or remain unchanged with different degrees of plasticity in the genotypes under N stress and correlated with reduced Fd-GOGAT activity, which supply the primary assimilate glutamate. GS2/Fd-GOGAT enzyme activity along with total N accumulation, protein, chlorophyll, and carotenoid content in shoot were found responsive to the N stress through combined PCA analysis. Our study confirmed the conserved nature of GS2 and Fd-GOGAT enzymes at the CDS and protein level; however, their expression and subsequent effects were different in cultivated wheat and their progenitors.Not Availabl