Implications of kinetically-hindered metals in ecotoxicological studies: Effect of platinum spike aging on its toxicity to Dunaliella salina

7 pages, 4 figures, 1 table.-- Under a Creative Commons licensePlatinum (Pt) is considered an emerging environmental micro-contaminant due to its increasing use in anthropogenic activities during the past decades. However, there are still important gaps in the understanding of its biogeochemical behavior in the aquatic environment – e.g. its speciation, reactivity and fate – mainly as a result of the analytical challenge of the determination of its typical ultra-trace environmental concentrations. Also, Pt is a kinetically-hindered metal displaying slow reaction kinetics, which has important implications regarding eco-toxicological studies. That is, investigation of its toxicity under laboratory-controlled conditions may therefore require ensuring that equilibrium speciation conditions are reached before starting the experiments. In order to shed further light on this issue, in this study we have monitored the speciation changes during aging of the Pt(IV) spikes in controlled media (seawater) using an UV-Vis spectrophotometry. Platinum toxicity to the green microalgae Dunaliella salina was then compared, using standardized tests, with fresh and aged Pt(IV) spikes at the mg L−1 concentration range. Following 96-hour exposure, ecotoxicological assays consisting in spectrometric measurements of chlorophyll-a concentrations and Effective Concentrations (EC) of Pt resulting in the inhibition of 10% and 50% of algae growth rate were calculated (EC10 and EC50, respectively). Daily monitoring of Pt speciation reflected the transition from PtCl62- (spike) to hydrolyzed species, probably in the form [PtCl3−n(OH)3+n]2-, n = 0–3. Exposure experiments showed that after a short period of aging (10 days), Pt(IV) toxicity increased one order of magnitude compared to freshly spiked media. These results confirm the relevance of considering spike aging to ensure that speciation equilibrium conditions are attained in order to produce environmental realistic eco-toxicological dataFinancial support from the Spanish Ministry of Science, Innovation and Universities (A. Romero-Freire contract, IJC2019-041160-I), and the Portuguese FCT (M. Abdou contract, CEECIND/01777/2018) are gratefully acknowledged. This manuscript is a contribution to the project ‘Technology-Critical Elements in Coastal Systems: Concentrations, Bioavailability, Trophic Transfer and Risk Assessment (TEC-RISK)’ (Ref. PID2019-111261GB-I00) funded by the Spanish Ministry of Science and InnovationPeer reviewe