A clonal cell line from immortalized olfactory ensheathing glia promotes functional recovery in the injured spinal cord

Immortalized cell lines of olfactory ensheathing glia (OEG) that maintain the proregenerative properties of primary cultures provide an unlimited source of OEG for both basic and applied studies. Indeed, one specific immortalized rat OEG clonal line (TEG3) proved to be as good as primary OEG in promoting neuritogenesis and axon regeneration in culture models. Thus, we examined the capacity of TEG3 to promote axonal repair in an animal model of spinal cord injury, dorsal column crush. TEG3 cells can acquire astrocyte-like or Schwann cell-like morphology depending on the conditions under which they are cultured. In the injured spinal cord, prelabeled TEG3 survived for at least 10 weeks after grafting and they integrated into the spinal cord, adopting Schwann cell-like, astrocyte-like, or intermediate morphologies. In TEG3-transplanted animals, sensory projection axons grow into the lesion site and there was robust sprouting/axonal growth of the corticospinal tract, both into and beyond the lesion site, after crushing of the spinal cord–dorsal columns. TEG3-transplanted animals also recovered sensory and motor function in tape removal and beam walking behavioral tests. These data indicate that certain immortalized cell lines derived from a single cell can maintain the regenerative properties of primary OEG.This work was supported by grants from Neuropharma, DGCYT, the Wellcome Trust, and the MRC and by an institutional grant from the Fundación Ramón Areces. M.T.M. was supported by contracts from the Spanish CSIC, Neuropharma, and the Fundación “Severo Ochoa” and was the recipient of an EMBO short-term fellowship. M.J.M. was supported by Neuropharma, M.A. by the BBSRC, and E.P. by an FPI fellowship of the Spanish Ministry.Peer reviewe