Changes in plasma glucagon and insulin associated with fasting in sea bass (Dicentrarchus labrax)

Juvenile sea bass (Dicentrarchus labrax) were fasted for 22 days and changes in plasma insulin, glucagon and glucose levels, as well as glycogen and protein content in liver and muscle were analyzed. Glucagon increased initially on the 4th day of fasting. The glucagon/insulin ratio (G/I) increased from a value of 0.11±0.02 (24h of fasting) to 0.21±0.05 (4th day of fasting). Thereafter, both glucagon and insulin levels decreased and remained at low concentrations until the 22nd day of fasting. Plasma glucose levels fell at the beginning of fasting, stabilized between the 4th and 8th day, and gradually declined during the rest of the experiment. There was a body weight loss of 15% and a significant decrease in both the hepatosomatic index and mesenteric fat. The decrease in the percentage of muscle proteins was not significant, while liver glycogen content showed a sharp decline.This work was partially supported by a F.P.I. grant from the Ministerio de Educación y Ciencia (M.E.C.) to I. Navarro and partially by a grant from C.I.C.Y.T. n ° 1-83 (M.E.C.). We would like to thank Dr. E.M. Plisestkaya for her valuable criticism of the paper. We would also like to thank L. McAllister for help with the English manuscript