Sulfo-glycosaminoglycans (sGAGs) are involved in the assembly of tau in at least a subpopulation of paired helical filaments (PHFs) in Alzheimer’s disease (AD). To further understand the role of sGAG molecules in the structure of PHFs, we isolated PHFs from patients with AD and treated them with heparinase. Immunoelectron microscopy and Western blotting (WB) were used later on to analyze the changes obtained. The heparinase treatment abolished Tau14 and AT8 immunodecoration (two N-terminal tau antibodies) and increased PHF-1 labeling (a C-terminal antibody). In addition, heparinase-treated filaments are more labile than control ones as demonstrated by sodium dodecyl sulfate-extraction and subsequent WB. In summary, our results demonstrate t...
Alzheimer’s disease (AD) is a neurodegenerative disorder, with extensive evidence implicating the mi...
A pathological signature of Alzheimer's disease (AD) is the formation of neurofibrillary tangles com...
Transcellular propagation of protein aggregate “seeds” has been proposed to mediate the progression ...
It is known from previous work that tau is the main component of paired helical filaments (PHFs) and...
AbstractIn a previous study [Wang et al. (1996) Nat. Med. 2, 871–875], Wang et al. found (i) that ab...
Alzheimer’s disease (AD) is the most common form of dementia, characterized by progressive loss of m...
Glycosaminoglycans (GAGs), including heparan sulfates and chondroitin sulfates, are major components...
Glycosaminoglycans (GAGs), including heparan sulfates and chondroitin sulfates, are major components...
Alzheimer's disease (AD) is the most common form of dementia, characterized by progressive loss of m...
Alzheimer's disease (AD) is characterized by extracellular cerebral accumulation of amyloid beta pep...
Proteoglycans are associated with all kinds of amyloid deposits in the human body. These complex mac...
Alzheimer's disease (AD) is characterized by pathological lesions such as amyloid-beta (Abeta) plaqu...
Glycosaminoglycans (GAGs) are linear polysaccharides whose disaccharide building blocks consist of a...
Alzheimer's disease (AD) is characterized by pathological lesions such as amyloid-beta (A beta) plaq...
Protein aggregation into dense filamentous inclusions is a characteristic feature of many etiologica...
Alzheimer’s disease (AD) is a neurodegenerative disorder, with extensive evidence implicating the mi...
A pathological signature of Alzheimer's disease (AD) is the formation of neurofibrillary tangles com...
Transcellular propagation of protein aggregate “seeds” has been proposed to mediate the progression ...
It is known from previous work that tau is the main component of paired helical filaments (PHFs) and...
AbstractIn a previous study [Wang et al. (1996) Nat. Med. 2, 871–875], Wang et al. found (i) that ab...
Alzheimer’s disease (AD) is the most common form of dementia, characterized by progressive loss of m...
Glycosaminoglycans (GAGs), including heparan sulfates and chondroitin sulfates, are major components...
Glycosaminoglycans (GAGs), including heparan sulfates and chondroitin sulfates, are major components...
Alzheimer's disease (AD) is the most common form of dementia, characterized by progressive loss of m...
Alzheimer's disease (AD) is characterized by extracellular cerebral accumulation of amyloid beta pep...
Proteoglycans are associated with all kinds of amyloid deposits in the human body. These complex mac...
Alzheimer's disease (AD) is characterized by pathological lesions such as amyloid-beta (Abeta) plaqu...
Glycosaminoglycans (GAGs) are linear polysaccharides whose disaccharide building blocks consist of a...
Alzheimer's disease (AD) is characterized by pathological lesions such as amyloid-beta (A beta) plaq...
Protein aggregation into dense filamentous inclusions is a characteristic feature of many etiologica...
Alzheimer’s disease (AD) is a neurodegenerative disorder, with extensive evidence implicating the mi...
A pathological signature of Alzheimer's disease (AD) is the formation of neurofibrillary tangles com...
Transcellular propagation of protein aggregate “seeds” has been proposed to mediate the progression ...