Microtubule-associated protein 1B is involved in the initial stages of axonogenesis in peripheral nervous system cultured neurons

Neuronal process extension is dependent on the reorganisation of the cytoskeleton, in particular microtubules and microfilaments, and one of the ways in which microtubules are regulated is by a group of microtubule-associated proteins called MAPs. MAP1B, the first MAP to be expressed in developing neurons, has been shown to play an important role during axonogenesis. Previously, we have shown that a phosphorylated isoform of MAP1B is involved in maintaining growth cone microtubules in a dynamically unstable state. In order to further investigate the role of MAP1B during axonogenesis we have cultured dorsal root ganglion (DRG) neurons from a MAP1B deficient mutant mouse. These mice express only trace amounts of MAP1B, have defects in the development of their nervous system and die perinatally. Cultured DRG neurons from MAP1B deficient mice show a reduction in axon elongation and an increase in growth cone area. The reduction in axon elongation is most likely to occur due to an inhibition in the early stages of axonogenesis. Using time-lapse video we have verified that during the first 2 h after plating, MAP1B deficient neurones extend their axons with an average speed that is half the speed of control neurones. These results support the participation of MAP1B during the initial stages of axonogenesis.Christian Gonzalez-Billault is supported by a Comunidad Autonoma de Madrid Postdoctoral Fellowship. Rebecca Owen is supported by a BBSRC studentship. The research described in this paper was made possible by an Aciones Integradas grant from the British Council and CSIC. The MAP1B research in Professor Gordon-Weeks’ laboratory is supported by The Wellcome Trust and by CICYT and Comunidad Autónoma de Madrid in Professor Avila’s laboratory.Peer reviewe