Protein composition analysis of oil bodies from maize embryos during germination

Seed oil bodies (OBs) are intracellular particles that store lipids. In maize embryos, the oil bodies are accumulated mainly in the scutellum. Oil bodies were purified from the scutellum of germinating maize seeds and the associated proteins were extracted and subjected to 2-DE analysis followed by LC–MS/MS for protein identification. In addition to the previously known oil body proteins oleosin, caleosin and steroleosin, new proteins were identified.This work was carried out with grants BIO2004-01577 and AGL2009-09151 from Plan Nacional de Investigación Científica y Técnica, by the Centre CONSOLIDER on Agrigenomics and by the Xarxa de Referencia en Biotecnologia of the Generalitat de Catalunya. H.T. was the recipient of a predoctoral I3P (CSIC).Peer reviewe