Application of Biochar Produced from Crop Residues on Trace Elements Contaminated Soils: Effects on Soil Properties, Enzymatic Activities and Brassica rapa Growth

18 páginas.- 5 figuras.- 5 tablas.- 58 referencias.- Supplementary Materials: The following are available online at https://www.mdpi.com/article/10.3390/agronomy11071394/s1Soil pollution by trace elements is a huge problem around the globe. In addition, heavy metal immobilization and primary productivity are two soil ecosystem services of contemporary importance to society. The goal of this study was to evaluate the effects of using olive pit and rice husk biochars as soil amendments for the immobilization of trace elements and on plant development growing in heavy metals-polluted soils under greenhouse conditions. The application of high doses (5% and 10%) of biochar significantly increased pH, water holding capacity and total C content of the soils. Dehydrogenase activity in the moderately acidic soil was greater than in the acidic soil due to the high concentration of metals and high acidity of the latter. The application of biochar reduced the β–glucosidase activity. Furthermore, the concentrations of CaCl2-extractable heavy metals significantly decreased in biochar amended pots, indicating metal immobilization, which was consistent with the increase in soil pH. Distribution of trace elements in the different fractions was modified after 65 days of incubation, independently of the treatment. The Cu and Zn contents in the oxidizable fraction were reduced with incubation, whereas Cd and Zn in the residual fraction increased. The reduction of bioavailable concentrations and increments in the residual or more stable fractions indicated less risk for the organisms in the environment. All biochars addition significantly increased the root-to-shoot ratio compared to the control soil. Particularly, 10% of amendment increased this ratio in the greatest extent. The application of 10% w/w of rice husk biochar produced at 500 °C was the most effective in restoring soil functionality and reducing the availability of heavy metals in the polluted soilsThis research was funded by The Spanish Ministry of Economy, Industry and Competitiveness (MINEICO) and AEI/FEDER [project CGL2016-76498-R]; MINEICO is also thanked for funding the “Ramón y Cajal” post-doctoral contract of José M. De la Rosa (ref: RYC2014-16338). P. Campos thanks the “Fundación Tatiana Pérez de Guzmán el Bueno” for funding her PhD. CSIC is thanked for funding the project 2020AEP095Peer reviewe