Photodynamic inactivation of microorganisms using (aza)phthalocyanine photosensitisers

Charles University Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Author: Bc. Pavlína Holmanová Supervisor: RNDr. Miloslav Macháček, Ph.D. Consultant: RNDr. Klára Konečná, Ph.D. Title of diploma thesis: Photodynamic inactivation of microorganisms using (aza)phthalocyanine photosensitisers Background: This work is aimed to optimize methodological approaches for the evaluation of the antimicrobial activity of selected representative (aza)phthalocyanine photosensitizers (PSs) in vitro. In addition, the in vitro antimicrobial activity of candidate PSs was evaluated against three clinically important pathogens. Namely, gram positive methicillin resistant bacterium Staphylococcus aureus (MRSA), gram negative bacterium Pseudomonas aeruginosa (PA), and yeast Candida albicans (CA) were included in the study. Methods: Microorganisms were incubated with the selected PSs, which were subsequently washed away, and microorganisms with bound PSs were irradiated for 15 or 30 min. At the same time, the intrinsic antimicrobial activities of the studied compounds without irradiation were determined as well. Three different methodological approaches were used to evaluate the minimum bactericidal concentration (MBC)/minimum fungicidal concentration (MFC). The Bioscreen C instrument was used for..