Optimization of the method for viability testing in Haemonchus contortus

Charles University Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Bc. Eva Mašátová Supervisor: prof. RNDr. Lenka Skálová, Ph.D. Consultant: Mgr. Linh Thuy Nguyen Title of diploma thesis: Optimization of the method for viability testing in Haemonchus contortus Barber's pole worm (Haemonchus contortus) is one of the most widespread pathogenic nematodes of the Trichostrongylidae family parasitizing in the abomasum of small ruminants, mainly in sheep. The most common symptoms of haemonchosis are anaemia, lack of thriving and weight loss. Young animals can also die. A significant complication in the treatment of haemonchosis is the rapidly developing resistance of the barber's pole worm to the administered drugs, which has become a global problem in recent years. This work focused on testing the efficacy of benzimidazole anthelmintics (thiabendazole and albendazole) in three strains of barber's pole worm, which differ in their drug sensitivity. We worked with strains: ISE (Inbred Susceptible Edinburgh) strain - sensitive, IRE (Inbred Resistant Edinburgh) strain - resistant and WR (White-River) strain - multi- resistant. The egg hatch test (EHT) was used to monitor the drug effectiveness and thus to monitor the level of resistance. Eggs from individual strains were..