Study of the effect of cosmetics on the human skin microbiome using molecular techniques

The theoretical part of the thesis is focused on the basic description of the physiology of the skin, human microbiome and a brief summary of where individual microorganisms occur. Furthermore, there is a list of analytical and microbiological methods that are used in this thesis. In the beginning, the practical part is focused on determination of antimicrobial effects of the chosen cosmetic products using microbial tests. Then, the inhibiton and microbial effect of the chosen cosmetic products on examined microorganisms has been measured using ELISA method. The second part of the thesis is focused on the isolation of bacterial DNA in quality that would be high enough to be used for amplification in PCR. There has been an optimalization of isolation of microbial DNA that was to be found on tested subjects’ skin. The presence of chosen microorganisms on skin before and after the usage of cosmetic products was measured using a PCR method. PCR products were then detected using gel electrophoresis. From the gathered data it is clear that the number of observed microorganisms has changed significantly after the application of cosmetic products