Modification of porosity of bacterial cellulose in situ

This bachelor thesis deals with the production and modification of porosity of bacterial cellulose in situ using the bacterium Komagataeibacter xylinus. The theoretical part of the work was focused on the review of various methods of culturing Komagataeibacter xylinus and the production of porous samples of bacterial cellulose. The sizes of pores in bacterial cellulose depend mainly on the applied cultivation method. Bacterial cellulose produced statically or dynamically contains pores with the dimensions of approximately 0.02 µm to 10 µm. The difference in porosity in bacterial cellulose prepared by static and dynamic cultivation was confirmed experimentally. The production yields of bacterial cellulose were compared and discussed. Next, the porosity of the bacterial cellulose was modified in situ by the addition of wax particles. Scanning electron microscopy confirmed, that the accumulation of wax particles in the production medium could significantly support the porosity of bacterial cellulose and, at the same time, increase its production