Preparation and characterization of hydrogels with diffusion gradient

The presented bachelor thesis deals with the preparation and further characterization of hydrogels with a diffusion gradient. The aim of the thesis is a literature research on the issue of hydrogels with a diffusion gradient, and a selection of a suitable method for its preparation. Moreover, conducted pilot experiments lead to the characterization of the properties of the prepared systems, especially with regard to proving the gradient structure of hydrogels. The performed evaluation was based on visual observation, determination of transport, and viscoelastic properties. In addition, a simple method consisting of weighing the prepared hydrogels was used. Hydrogels were compared depending on the choice of crosslinking agent, its concentration, and crosslinking time. Gradient hydrogels based on polysaccharide sodium alginate were prepared. The results suggest that by selecting the concentration of the crosslinking agent, as well as the crosslinking time, the final properties (viscoelastic and transport) of the hydrogels can be adjusted to the desired values. In the discussion of the results, we propose steps for a more comprehensive view of the issue and providing options for the following research