Alkaloids screening with a focus on potential drug discovery

Charles University, Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical Botany Candidate: Mgr. Leona Adamičková Supervisor: Prof. RNDr. Lubomír Opletal, CSc. Title of Rigorous Thesis: Alkaloids screening with a focus on potential drug discovery Key words: cholinesterase inhibition, Alzheimer disease, plant extract Within new anti-Alzheimer agent searching a screening of inhibition activity of various plants was performed. For this study plants used in traditional medicine were chosen and alkaloidal extracts were prepared and their potential to human cholinesterases inhibition was determined. For extraction bulbs of Fritillaria ussurensis, bulbs of F. cirrhosis, cortex and flowers of Magnolia officinalis, flowers of M. biondii, Nelumbo nucifera seeds, leaves and roots with rhizomes of Nuphar luteum, Papaver rheum roots, Laurus nobilis fruits and seeds of Ziziphus jujuba var. spinosa were used. The cholinesterase inhibitory activity was determined in vitro by modified Ellman's method. Extract of Fritillaria ussurensis bulbs was found to be the most potent inhibitor of BuChE with IC50 value of 11,63 ± 3,7 μg/mL. Extract of M. biondii inhibited both cholinesteras with IC50 values lower than 50 μg/mL. Among extracts with inhibition activity to BuChE pertain extracts of F. cirrhosa bulbs,..