Determination of creatinine in urine using on-line SPE in SIA

Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Analytical chemistry Candidate: Bc. Eva Semerádová Supervisor: Assoc. Prof. PharmDr. Petr Chocholouš, Ph.D. Title of the diploma thesis: Determination of creatinine in urine using on-line SPE in SIA The aim of the diploma thesis was to determine creatinine in urine by a method whose results would be comparable to the routinely used method based on the Jaffé reaction. Three urine samples were used, the resulting values were statistically compared with the values measured on a clinical analyzer according to the Jaffé protocol. Urine creatinine was determined by online solid phase extraction (SPE) in sequence injection analysis (SIA). The column in the SIA system was filled with sorbent for extraction, a sorbent was used in the Plexa PCX material. The standard addition method was used to determine creatinine, each sample was measured twice and the average of the given values was used for evaluation. The experimental conditions were first optimized on standard solutions in dilute acetic acid, then applied to real urine samples. The method was evaluated for linearity, yield, repeatability and robustness. According to the value of the correlation coefficient, good linearity was achieved, yield around 100%. Repeatability was..