Oxidative stress plays a critical role in the pathogenesis of a number of diseases. The carbonyl end products of protein oxidation are among the most commonly measured markers of oxidation in biological samples. Protein carbonyl functional groups may be derivatized with 2,4-dinitrophenylhydrazine (DNPH) to render a stable 2,4-dinitrophenylhydrazone-protein (DNP-protein) and the carbonyl contents of individual proteins then determined by two-dimensional electrophoresis followed by immunoblotting using specific anti-DNP antibodies. Unfortunately, derivatization of proteins with DNPH could affect their mass spectrometry (MS) identification. This problem can be overcome using nontreated samples for protein identification. Nevertheless, derivati...
Most of the assays for detection of carbonylated proteins, the most general and widely used marker o...
Protein carbonylation, a marker of excessive oxidative stress, has been studied in the context of mu...
Among disruptions induced by oxidative stress, modifications of proteins, particularly irreversible ...
Several studies demonstrated the involvement of free radicals in the pathophysiology of neurodegener...
Protein carbonylation represents the most frequent and usually irreversible oxidative modification a...
20 pages, 1 table, 2 figuresCarbonylation is a nonenzymatic irreversible posttranslational protein m...
Oxidative stress, an imbalance toward the pro-oxidant side of the pro-oxidant/antioxidant homeostasi...
AbstractProtein oxidation is involved in regulatory physiological events as well as in damage to tis...
International audienceIncreased protein carbonyl content is a hallmark of cellular and organismal ag...
Most of the assays for detection of carbonylated proteins, the most general and widely used marker o...
<p>Top: 2D maps of CTR (left) and DYS (right) cervical tissues. Proteins (300 µg) were separated in ...
The data reported here are a comparison among four different methods for the detection of carbonylat...
<p>Top: 2D gel maps of DYS (right) and SCC (left) cervical tissues. Protein (300 µg) were separated ...
Mass spectrometry (MS) is an analytical technique allowing the investigation of a single protein or ...
International audienceProtein carbonyls (PC) are oxidative damage observed in many diseases. Protein...
Most of the assays for detection of carbonylated proteins, the most general and widely used marker o...
Protein carbonylation, a marker of excessive oxidative stress, has been studied in the context of mu...
Among disruptions induced by oxidative stress, modifications of proteins, particularly irreversible ...
Several studies demonstrated the involvement of free radicals in the pathophysiology of neurodegener...
Protein carbonylation represents the most frequent and usually irreversible oxidative modification a...
20 pages, 1 table, 2 figuresCarbonylation is a nonenzymatic irreversible posttranslational protein m...
Oxidative stress, an imbalance toward the pro-oxidant side of the pro-oxidant/antioxidant homeostasi...
AbstractProtein oxidation is involved in regulatory physiological events as well as in damage to tis...
International audienceIncreased protein carbonyl content is a hallmark of cellular and organismal ag...
Most of the assays for detection of carbonylated proteins, the most general and widely used marker o...
<p>Top: 2D maps of CTR (left) and DYS (right) cervical tissues. Proteins (300 µg) were separated in ...
The data reported here are a comparison among four different methods for the detection of carbonylat...
<p>Top: 2D gel maps of DYS (right) and SCC (left) cervical tissues. Protein (300 µg) were separated ...
Mass spectrometry (MS) is an analytical technique allowing the investigation of a single protein or ...
International audienceProtein carbonyls (PC) are oxidative damage observed in many diseases. Protein...
Most of the assays for detection of carbonylated proteins, the most general and widely used marker o...
Protein carbonylation, a marker of excessive oxidative stress, has been studied in the context of mu...
Among disruptions induced by oxidative stress, modifications of proteins, particularly irreversible ...