Enrichment and Detection of Antigen-Binding B Cells for Mass Cytometry

Zachary C. Stensland
Mia J. Smith

Open link

Publication date

June 2021

DOI

10.3390/magnetochemistry7070092

Publisher

MDPI AG

Journal

Magnetochemistry

Abstract

Over the years, various techniques have been utilized to study the function and phenotype of antigen-binding B cells in the primary repertoire following immunization, infection, and development of autoimmunity. Due to the low frequency of antigen-reactive B cells (<0.05% of lymphocytes) in the periphery, preliminary enrichment of cells is necessary to achieve sufficient numbers for statistically sound characterization, especially when downstream analytic platform use, e.g., CyTOF, is low throughput. We previously described a method to detect and enrich antigen-reactive B cells from peripheral blood and tissues using biotinylated antigens in conjunction with magnetic nanoparticles, preparative to a downstream analysis by ELISPOT and flow cyt...