Injectable anti-infectious wound dressing hydrogel using marine poly, and oligo-saccharide

Given advances in the treatment of various diseases such as cancer, where specific treatment for targeted tissues or polytherapy is common, our toolkit for wound healing is relatively empty. There is an immense need for innovative biomaterials to address the acute inflammation and antibiotic-resistant organisms in infected wounds. We developed anti-infectious, injectable wound dressing hydrogels with antibacterial and antioxidant activity derived from chitooligosaccharides (COS) as bioactive compounds (developed in our lab) for chronic skin wounds. A co-enzymatically mediated crosslinking using horseradish (HRP) and glucose oxidase (GOx) triggered by glucose lead to a gradual release of hydrogen peroxide (H2O2) required for the activation of HRP, resulted in a mild enzymatic crosslinking of the phenol containing polysaccharides (chitosan, and alginate). GOx consumes the glucose and gradually releases H2O2 resulting in the activation of HRP and initializing the crosslinking; besides, glucono-delta-lactone (GDL), as a byproduct of the glucose oxidation reaction, is hydrolyzed and gradually donates protons, leading to the protonation of more free amino groups of chitosan and COS, which results in the enhancement of the electrostatic interactions with negatively charged alginate. The physiochemical investigation of the hydrogel revealed a suitable swelling ratio (