The measurement of changes in nicotinic acid adenine dinucleotide phosphate (NAADP) levels in cells has been, and remains, key to the investigation of the functions of NAADP as a Ca2+-releasing second messenger. Here we provide details of how to isolate NAADP from cells by extraction with perchloric acid and then measure the NAADP using a radioreceptor assay. We demonstrate that NAADP is neither generated nor broken down during sample processing conditions and that radioreceptor assay is highly selective for the detection of NAADP under cell extract conditions. Furthermore, a number of improvements, such as solid-state detection of the radioactivity, are incorporated to enhance the safety of the procedure. Finally, we have developed a new m...
Cyclic ADP-ribose (cADPR) and nicotinic acid adenine dinucleotide phosphate (NAADP) are two novel Ca...
Nicotinic acid adenine dinucleotide phosphate (NAADP) is a potent activator of intracellular Ca2+ re...
Nicotinic acid adenine dinucleotide phosphate (NAADP) is a second messenger that releases Ca2+ from ...
AbstractThe measurement of changes in nicotinic acid adenine dinucleotide phosphate (NAADP) levels i...
The measurement of changes in nicotinic acid adenine dinucleotide phosphate (NAADP) levels in cells ...
Nicotinic acid-adenine dinucleotide phosphate (NAADP) is a novel nucleotide derived from NADP that h...
SummaryNicotinic acid adenine dinucleotide phosphate (NAADP, 1) is the most potent intracellular Ca2...
Nicotinic acid adenine dinucleotide phosphate (NAADP) is a metabolite of NADP with Ca2+ mobilizing a...
Nicotinic acid adenine dinucleotide phosphate (NAADP) is a potent intracellular second messenger cap...
NAADP (nicotinic acid adenine dinucleotide phosphate) is a recently discovered second messenger, and...
Research into the biological role of the Ca2+-releasing second messenger NAADP (nicotinic acid adeni...
Nicotinic acid adenine dinucleotide phosphate (NAADP) mobilizes Ca2+ through a mechanism totally ind...
Nicotinic acid adenine dinucleotide phosphate (NAADP) is struc-turally related to the well-known coe...
Nicotinic acid adenine dinucleotide phosphate (NAADP) mobilizes Ca2+ through a mechanism totally ind...
Nicotinamide adenine dinucleotide (NAD), a prominent member of the pyridine nucleotide family, plays...
Cyclic ADP-ribose (cADPR) and nicotinic acid adenine dinucleotide phosphate (NAADP) are two novel Ca...
Nicotinic acid adenine dinucleotide phosphate (NAADP) is a potent activator of intracellular Ca2+ re...
Nicotinic acid adenine dinucleotide phosphate (NAADP) is a second messenger that releases Ca2+ from ...
AbstractThe measurement of changes in nicotinic acid adenine dinucleotide phosphate (NAADP) levels i...
The measurement of changes in nicotinic acid adenine dinucleotide phosphate (NAADP) levels in cells ...
Nicotinic acid-adenine dinucleotide phosphate (NAADP) is a novel nucleotide derived from NADP that h...
SummaryNicotinic acid adenine dinucleotide phosphate (NAADP, 1) is the most potent intracellular Ca2...
Nicotinic acid adenine dinucleotide phosphate (NAADP) is a metabolite of NADP with Ca2+ mobilizing a...
Nicotinic acid adenine dinucleotide phosphate (NAADP) is a potent intracellular second messenger cap...
NAADP (nicotinic acid adenine dinucleotide phosphate) is a recently discovered second messenger, and...
Research into the biological role of the Ca2+-releasing second messenger NAADP (nicotinic acid adeni...
Nicotinic acid adenine dinucleotide phosphate (NAADP) mobilizes Ca2+ through a mechanism totally ind...
Nicotinic acid adenine dinucleotide phosphate (NAADP) is struc-turally related to the well-known coe...
Nicotinic acid adenine dinucleotide phosphate (NAADP) mobilizes Ca2+ through a mechanism totally ind...
Nicotinamide adenine dinucleotide (NAD), a prominent member of the pyridine nucleotide family, plays...
Cyclic ADP-ribose (cADPR) and nicotinic acid adenine dinucleotide phosphate (NAADP) are two novel Ca...
Nicotinic acid adenine dinucleotide phosphate (NAADP) is a potent activator of intracellular Ca2+ re...
Nicotinic acid adenine dinucleotide phosphate (NAADP) is a second messenger that releases Ca2+ from ...