Analysis of the role of Sgs1p in repair of double-strand DNA breaks in \u3ci\u3eSaccharomyces cerevisiae\u3c/i\u3e

Mis-repair of DNA double-stranded breaks (DSB) can result in genomic rearrangements and cancer. Sgs1p is a helicase that unwinds DNA for DSB repair. To better understand its role in break repair, I analyzed the frequency of various types of DSB repairs in yeast cells with the mutation sgs1-FD. Given this mutation disrupts an interaction between Sgs1p and a critical protein, Rad51p, I hypothesized mutant cells would have more error-prone repair than high fidelity canonical BIR repair. Genome sequence and chromosome size were examined in sgs1-FD yeast cells that underwent DSB repair. No difference was found in the frequency of error-prone repair, indicating the mutation did not affect the repair process