Thesis (Ph.D.)--University of Washington, 2015-12Human cytomegalovirus (HCMV) lacking TRS1 and IRS1 (HCMV[ΔI/ΔT]) cannot replicate in cell culture. Although both proteins can block the protein kinase R (PKR) pathway, they have been reported to have multiple other activities and binding partners. It remains unknown which of these functions are essential for HCMV replication. To investigate this issue, we first identified a TRS1 mutant that is unable to bind to PKR. Like HCMV[ΔI/ΔT], a recombinant HCMV containing this mutant did not replicate in wild-type cells. However, HCMV[ΔI/ΔT] did replicate in cells in which PKR expression was reduced by RNA interference. Moreover, HCMV[ΔI/ΔT] and the recombinant mutant replicated to similar levels as v...
Many viruses have evolved mechanisms to evade the repression of translation mediated by protein kina...
Human Cytomegalovirus (HCMV) is a widespread pathogen that causes lifelong latent infection. HCMV ra...
Replication of human cytomegalovirus (HCMV) is characterized by a tight virus-host cell interaction....
Thesis (Ph.D.)--University of Washington, 2015-12Human cytomegalovirus (HCMV) lacking TRS1 and IRS1 ...
AbstractHuman cytomegalovirus (HCMV) lacking TRS1 and IRS1 (HCMV[ΔI/ΔT]) cannot replicate in cell cu...
AbstractProtein Kinase R (PKR) inhibits translation initiation following double-stranded RNA (dsRNA)...
Thesis (Ph.D.)--University of Washington, 2012Double-stranded RNA (dsRNA) that accumulates during ma...
Cytomegaloviruses (CMVs) are generally unable to cross species barriers, in part because prolonged c...
ABSTRACT Human cytomegalovirus (HCMV) counteracts host defenses that otherwise act to limit viral pr...
Thesis (Ph.D.)--University of Washington, 2016-11During millions of years of coevolution with their ...
ABSTRACT Double-stranded RNAs (dsRNA) produced during human cytomegalovirus (HCMV) infection activat...
Decades of research on vaccinia virus (VACV) have provided a wealth of insights and tools that have ...
The replication of human cytomegalovirus (HCMV) is characterized by a complex network of virus–host ...
The complex host interaction network of human cytomegalovirus (HCMV) involves the regulatory protein...
The UL97-encoded protein kinase (pUL97) of human cytomegalovirus (HCMV) plays a critical role in the...
Many viruses have evolved mechanisms to evade the repression of translation mediated by protein kina...
Human Cytomegalovirus (HCMV) is a widespread pathogen that causes lifelong latent infection. HCMV ra...
Replication of human cytomegalovirus (HCMV) is characterized by a tight virus-host cell interaction....
Thesis (Ph.D.)--University of Washington, 2015-12Human cytomegalovirus (HCMV) lacking TRS1 and IRS1 ...
AbstractHuman cytomegalovirus (HCMV) lacking TRS1 and IRS1 (HCMV[ΔI/ΔT]) cannot replicate in cell cu...
AbstractProtein Kinase R (PKR) inhibits translation initiation following double-stranded RNA (dsRNA)...
Thesis (Ph.D.)--University of Washington, 2012Double-stranded RNA (dsRNA) that accumulates during ma...
Cytomegaloviruses (CMVs) are generally unable to cross species barriers, in part because prolonged c...
ABSTRACT Human cytomegalovirus (HCMV) counteracts host defenses that otherwise act to limit viral pr...
Thesis (Ph.D.)--University of Washington, 2016-11During millions of years of coevolution with their ...
ABSTRACT Double-stranded RNAs (dsRNA) produced during human cytomegalovirus (HCMV) infection activat...
Decades of research on vaccinia virus (VACV) have provided a wealth of insights and tools that have ...
The replication of human cytomegalovirus (HCMV) is characterized by a complex network of virus–host ...
The complex host interaction network of human cytomegalovirus (HCMV) involves the regulatory protein...
The UL97-encoded protein kinase (pUL97) of human cytomegalovirus (HCMV) plays a critical role in the...
Many viruses have evolved mechanisms to evade the repression of translation mediated by protein kina...
Human Cytomegalovirus (HCMV) is a widespread pathogen that causes lifelong latent infection. HCMV ra...
Replication of human cytomegalovirus (HCMV) is characterized by a tight virus-host cell interaction....