Thesis (Master's)--University of Washington, 2014Current newborn screening programs for phenylketonuria (PKU) do not reach many individuals, worldwide, who would benefit from testing. Existing PKU tests require a high-resource laboratory, as well as a base level of local infrastructure, which prohibits their use in low-resource settings. In this report, we demonstrate a paper-based PKU test, based on a well-known enzyme colorimetric assay (ECA), which is applicable to low-resource settings. Phenylalanine dehydrogenase (PheDH) catalyzes the reaction between phenylalane (Phe) and NAD+ to produce phenylpyruvate, NH4, and NADH. Subsequently, NADH reacts with nitroblue tetrazolium salt (NBT) and phenazine methosulfate (PMS), an intermediate elec...
Phenylalanine (Phe) is the most reliable indicator for the diagnosis of phenylketonuria (PKU). The p...
Introduction: Inborn errors of metabolism (IEM) are a collective group of rare genetic disorders tha...
Detection of individuals with phenylketonuria (PKU), an autosomal recessively inherited disorder in ...
Graduation date: 2016Phenylketonuria (PKU) is a genetic inborn metabolic disorder which inhibits\ud ...
Introduction: The PKU is an inborn error of amino acid metabolism, in which phenylalanine (Phe) accu...
Abstract Phenylalanine analysis for phenylketonuria (PKU) detection in newborn screening (NBS) was c...
This is the publisher’s final pdf. The published article is copyrighted by the author(s) and publish...
Summary: A fluorometric assay in microtitre plates for the screening of phenylketonuria was evaluate...
The Quantase R Phenylalanine Assay, an enzymatic/colorimetric method for the determination of phenyl...
Neonatal screening for phenylketonuria (PKU, OMIM: 261600) was introduced at the end of the 1960s. W...
Phenylketonuria (PKU) was the first disease to be identified by the newborn screening (NBS) program....
Phenylketonuria (PKU) is commonly included in the newborn screening panel of most countries, with va...
The fluorometric analysis of phenylalanine described by McCaman and Robins has been adapted to autom...
A method is described for a spectrophotofluorometricdetermination of phenylalanine. The amino acid i...
Phenylketonuria (PKU) is caused by deficient activity of phenylalanine hydroxylase (PAH), responsibl...
Phenylalanine (Phe) is the most reliable indicator for the diagnosis of phenylketonuria (PKU). The p...
Introduction: Inborn errors of metabolism (IEM) are a collective group of rare genetic disorders tha...
Detection of individuals with phenylketonuria (PKU), an autosomal recessively inherited disorder in ...
Graduation date: 2016Phenylketonuria (PKU) is a genetic inborn metabolic disorder which inhibits\ud ...
Introduction: The PKU is an inborn error of amino acid metabolism, in which phenylalanine (Phe) accu...
Abstract Phenylalanine analysis for phenylketonuria (PKU) detection in newborn screening (NBS) was c...
This is the publisher’s final pdf. The published article is copyrighted by the author(s) and publish...
Summary: A fluorometric assay in microtitre plates for the screening of phenylketonuria was evaluate...
The Quantase R Phenylalanine Assay, an enzymatic/colorimetric method for the determination of phenyl...
Neonatal screening for phenylketonuria (PKU, OMIM: 261600) was introduced at the end of the 1960s. W...
Phenylketonuria (PKU) was the first disease to be identified by the newborn screening (NBS) program....
Phenylketonuria (PKU) is commonly included in the newborn screening panel of most countries, with va...
The fluorometric analysis of phenylalanine described by McCaman and Robins has been adapted to autom...
A method is described for a spectrophotofluorometricdetermination of phenylalanine. The amino acid i...
Phenylketonuria (PKU) is caused by deficient activity of phenylalanine hydroxylase (PAH), responsibl...
Phenylalanine (Phe) is the most reliable indicator for the diagnosis of phenylketonuria (PKU). The p...
Introduction: Inborn errors of metabolism (IEM) are a collective group of rare genetic disorders tha...
Detection of individuals with phenylketonuria (PKU), an autosomal recessively inherited disorder in ...