Synthesis, Characterization, and Nanodisc Formation of Non‐ionic Polymers**

Although lipid nanodiscs are increasingly used in the structural studies of membrane proteins, drug delivery and other applications, the interaction between the nanodisc belt and the protein to be reconstituted is a major limitation. To overcome this limitation and to further broaden the scope of nanodiscs, a family of non‐ionic amphiphilic polymers synthesized by hydrophobic functionalization of fructo‐oligosaccharides/inulin is reported. We show the stability of lipid nanodiscs formed by these polymers against pH and divalent metal ions, and their magnetic‐alignment properties. The reported results also demonstrate that the non‐ionic polymers extract membrane proteins with unprecedented efficiency.Hydrophobically functionalized fructo‐oligosaccharides/inulin polymers were synthesized and demonstrated to form lipid bilayer nanodiscs. These non‐ionic amphiphilic polymer‐based nanodiscs are stable against pH and divalent metal ions and they align magnetically in the presence of an external magnetic field. The reported results also demonstrate that the non‐ionic polymers extract membrane proteins with unprecedented efficiency.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/168430/1/anie202101950_am.pdfhttp://deepblue.lib.umich.edu/bitstream/2027.42/168430/2/anie202101950-sup-0001-misc_information.pdfhttp://deepblue.lib.umich.edu/bitstream/2027.42/168430/3/anie202101950.pd