Extraction of synthetic LH RH from human urine

peer reviewedA method of extraction of synthetic LH-RH is studied in human urines, using porous glass (Spherosil) and methanol. In the defined conditions the yield is greater than 80%. It appears that the method is reproducible. The recovery varies essentially with the quantity of Spherosil and the pH of methanol. The use of methanol acidified at pH 3 increases the speed and the importance of labelled LH-RH recovery