Analytical and antimicrobial study of Vanga Bhasma with special reference to Ayurved Prakash

Background: Bhasma, the Ayurvedic organometallic preparation, is an incinerated metal or mineral prepared after several rounds of processing through Puta. Vanga Bhasma is also a novel preparation routinely prescribed for Prameha and genito-urinary disorders. Objectives: The present study deals with the preparation of Vanga Bhasma according to the procedures mentioned in Ayurved Prakash. The synthesized Bhasma samples were characterized by various analytical techniques. The Antimicrobial effects of these samples were studied against certain Gram +ve, Gram –ve and fungal organisms. Materials and Methods: The different steps involved in the synthesis of Vanga Bhasma include Shodhan, Jaran, Bhavana and Maran. Bhasma was incinerated by the traditional method of heating i.e., Puta. The obtained samples were analyzed for the quality control checks, on the parameters described in Ayurvedic texts as well as modern techniques such as SEM, EDX and XRD to find out the nature of the Vanga Bhasma samples. The anti-microbial study was done to find out the anti-microbial efficacy of the Vanga Bhasma samples. Results and Conclusions: This study reveals that the synthesized Bhasma was converted into its nontoxic oxide form and had a highly reduced particle size observed from SEM images. Average 13 Puta are required to prepare Vanga Bhasma which is grayish white coloured. Vanga Bhasma showed antimicrobial activity in inhibiting the growth of Staphylococcus aureus, Bacillus subtilis, Klebsiella pneumonia, Escherichia coli and Candida albicans with a concentration of 100mg/ml