IN VITRO TEST OF ANTIBACTERIAL ACTIVITY OF SHELL AND RUBBER SEED EXTRACTS AGAINST Aeromonas hydrophila

Catfish is one of leading fishery commodity in Indonesia that nowadays is using intensive technology. One of the problems in cultivating catfish is the invasion of bacteria Aeromonas hyprophila which can cause Motile Aetomonas Septicemia disease. Information regarding the antibacterial activity of shell and rubber seed extract on Aeromonas hydrophila is not yet known. Therefore it is necessary to do a research about the antibacterial activities of the shell and rubber seed extract against the pathogenic bacteria, Aeromonas hydrophila. This research method was sample preparation, extraction, phytochemical test, preparation of bacterial suspension, antibacterial test with agar diffusion method, minimum inhibitory concentration test. The result shows that the rubber seed shell extract contains antibacterial compounds such as saponin, terpenoids and flavonoids.  While the rubber seed extract contains terpenoid and flavonoid compounds. The shell and rubber seed extract at a concentration of 0.5-4 mg/mL showed very weak inhibition.Ikan lele merupakan salah satu komoditas perikanan unggulan di Indonesia yang saat ini banyak menggunakan teknologi budidaya secara intensif. Salah satu masalah dalam budidaya lele adalah serangan bakteri Aeromonas hydrophila yang dapat menyebabkan penyakit Motile Aeromonas Septicemia. Informasi mengenai aktivitas antibakteri Aeromonas hydrophila ekstrak cangkang dan biji karet belum diketahui, oleh karena itu perlu dilakukan penelitian mengenai aktivitas antibakteri ekstrak cangkang dan biji karet terhadap bakteri pathogen Aeromonas hydrophila. Metode penelitian ini meliputi persiapan sampel, ekstraksi, uji fitokimia, persiapan suspensi bakteri, uji antibakteri dengan metode difusi agar, uji Minimum Inhibitory Concentration. Hasil penelitian menunjukkan bahwa ekstrak cangkang biji karet mengadung senyawa antibakteri saponin, terpenoid dan flavonoid. Sedangkan ekstrak biji karet mengandung senyawa terpenoid dan flavonoid. Ekstrak cangkang dan biji karet pada konsentrasi 0,5-4 mg/mL menunjukkan daya hambat yang sangat lemah.     &nbsp