Red blood cell image enhancement techniques for cells with overlapping condition

This paper proposes an automatic algorithm consists of techniques that can separateoverlapping RBC for enhancing RBC image to improve counting precision. The proposedalgorithm is able to automatically count RBC of colour blood smear images based on thequality or challenging conditions of the images such as poor illumination of blood smear andmost importantly overlapping RBC. The algorithm comprises of two RBC segmentation thatcan be selected based on the image quality, circle mask technique and grayscale blood smearimage processing. Detail explanations including experimental results that show the effectiveness of the proposed techniques are described in this paper. The proposed algorithm has been successfully detect and separate agglomerate RBC with 96% accuracy. Algorithmvalidation was verified with RBC distribution area and confusion matrix.Keywords: red blood cell (RBC); complete blood count (CBC); automated segmentation;overlap RBC