Isolation, characterization and extracellular enzyme detection of microbial isolates from deteriorated apple (malus domestica) fruits

Studies were carried out on deteriorated apples obtained from the Just Rite supermarket, Ota, Ogun State, Nigeria, to isolate microorganisms associated with post-harvest deterioration of apple (Malus domestica) fruits. The bacterial species were identified using microscopy, morphology and various biochemical tests while macroscopy and morphology was used to identify the fungi isolated. The ability of the isolate to elaborate extracellular amylase and protease were tested for. Results revealed Aspergillus niger and Aspergillus flavus as the fungal species while the bacterial species isolated were Bacillus spp and Micrococcus spp. All the isolates except Aspergillus niger produced amylase in substantial amount. Micrococcus spp, Aspergillus niger and Aspergillus flavus produced protease. The results of this investigation if combined with further studies can be used in identifying organisms which could be used as a biological method in the control of apple pathogens.Keywords: Aspergillus niger, Aspergillus flavus, Bacillus spp, Micrococcus spp, agar