Phytochemical and antimicrobial study on the leaf extracts of Erythrophleum africanum (Caesalpiniaceae)

The leaf of Erythrophleum africanum was exhaustively extracted with ethanol using cold maceration techniques. This was subsequently partitioned with petroleum ether, chloroform, ethylacetate and nbutanol. The agar diffusion method was used to determine the antimicrobial activity against the following micro-organisms eithicillin resistant Staphylococus, Staphylococcus aureaus, Streptococcus feacalis, Escherichia coli, Pseudomonas aeruginosa, Salmonella typhi, Proteus vulgaris, Candida albicans, Candida krusei and Candida tropicalis. Minimum Inhibitory Concentration (MIC), minimum bactericidal concentration (MBC) and minimum fungicidal concentration (MFC) were determined. The in vitro antimicrobial screening revealed that the extracts exhibited diverse activities against different microbes with zones of inhibition ranging from 12 to 36 mm, MIC ranging from 3.25 to 60 mg/ml and MBC/MFC of 3.25 to 60 mg/ml for sensitive organisms at the tested concentrations. The activities observed could be attributed to the presence of terpenoids, saponins, flavonoids, alkaloids and tannins. The results justify the ethnomedicinal use of this plant in the treatment of sores, boils, wounds, dysentery, diarrhea and sexually transmitted infections.Keywords: Erythrophleum africanum, phytochemistry, antimicrobial activityAfrican Journal of Biotechnology, Vol. 13(4), pp. 598-603, 22 January, 201