Molecular cloning and characterization of a cDNA encoding cycloartenol synthase from Fritillaria thunbergii Miq.
- Zhu, Z-H
- Liu, W-H
- Ge, L-J
- Yu, Q
- Zhao, W-C
- Yang, J-H
DOIAbstract
Fritillaria thunbergii Miq., known as the bulbous plants of the genus fritillaria, produces a large amount of sterols. Homology based polymerase chain reactions (PCRs) with degenerate primers designed from the conserved sequences among the known cycloartenol synthase (CAS) resulted in cloning of a CAS from the young leaves of F. thunbergii Miq. A putative cycloartenol synthase cDNA (FtCAS) consists of a 2271 bp open reading frame, which encodes for 756 amino acids. The deduced amino acid sequence shows 82% homology to cycloartenol synthases from Dioscorea zingiberensis. Heterologous expression of the FtCAS in the methylotrophic yeast, Pichia pastoris, resulted in production of cycloartenol. Our results indicate that FtCAS encode cycloarteno...
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