Micropropagation and production of eurycomanone, 9- methoxycanthin-6-one and canthin-6-one in roots of Eurycoma longifolia plantlets

Eurycoma longifolia or Tongkat Ali is a well known medicinal plant in Malaysia. The plants are used as main portion in herbal preparation, and have been extensively harvested. Generally, most of the raw materials required by the industries are heavily collected from the natural forests, thereby resulting in the uncontrolled exploitation of the plant in the wild. Hence, there is a need to ensure adequate supply of quality E. longifolia for the use of the related industries and for conservation purposes. Micropropagation has proved to be an alternative for the multiplication of medicinal and aromatic plants and it can allow the future commercial use of E. longifolia in the herbal industries. In this study, E. longifolia plantlets were successfully mass-produced using axillary shoot multiplication techniques, and the production of eurycomanone, 9-methoxycanthin-6-one and canthin-6-one compounds of E. longifolia were detected in roots of tissue culture plantlets.Key words: E. longifolia, Tongkat Ali, micropropagation, eurycomanone, 9-methoxycanthin-6-one, canthin-6- one