Agrobacterium-mediated transient expression system in banana immature fruits

Transient expression assays are useful for saving time in studies of gene function, particularly ones expressed in fruit. Transient expression system of a reporter gene in immature fruit of banana was developed.Agrobacterium tumefaciens, whose plasmid contains an intron-containing Agrobacterium tumefaciens, whose plasmid contains an intron-containing Agrobacterium tumefaciens, whose plasmid contains an intron-containing -glucuronidase (gusA) gene under regulatory control of the CaMV 35S promoter, was vacuum-infiltrated into sliced fruits and co-cultured. GUS histochemical assay was performed three days after co-cultivation, and a high level of GUS expression was observed. This transient expression system is useful for routine transient assays to validate genes expressed in banana fruit