Bacteriocinogenicity and production of pyocins from Pseudomonas species isolated in Lagos, Nigeria

A total of 52 strains of Pseudomonas identified as P. aeruginosa (27), P. putida (9), P. fluorescens (13) and P. stutzeri (3) were isolated from Nigerian patients with burns, wound and skin infections, UTI,diarrhoea and eye infections. 81.5, 55.6, 76.9 and 33.3% of these species produced pyocins at a range of 410 – 670 g/ml. High yields of pyocins at 35 – 37oC, which declined sharply at temperatures above37oC were obtained from all the producing strains. The strains also displayed significant pyocin expression (P 3 mm. These pyocins also displayed growth inhibitory activity against some Nigerian isolates of Gram-positive and Gram-negative bacteria when undiluted and at 1:2 - 1:16 dilutions. The susceptible organisms include Bacillus cereus, Listeria monocytogenes, Klebsiella spp., Staphylococcus aureus, S. epidermidis,Proteus spp. and Vibrio parahaemolyticus. The results of this study have provided evidence for broadspectrum antibacterial activity of pyocins elicited by Pseudomonas species from Nigeri