Monitoring of Antioxidant and Antidiabetic Activity of Fractions from Ethyl Acetate Extract in Garcinia latissima Miq. Stem Bark

The genus Garcinia (Fam. Cluciaciae) is known as a source of phenolic compounds which are reported to have biological activities: antioxidants, antidiabetic, and anticancer etc. In this study, the monitoring was carried out for the antioxidant and antidiabetic activity of a number of fractions from the ethyl acetate extract of stem bark G. latissima Miq. (endemic plants, the Maluku Islands, Indonesia). The extraction process uses the maceration method with ethyl acetate solvent produced 240 g extract from 2 kg from dry bark of G. latissima Miq. Furthermore, the ethyl acetate extract was carried out by fractionation by Vacuum Liquid Chromatography (VLC) over the silica gel column and eluted using a mixture of n-hexane and ethyl acetate, with increasing polarity, and were obtained nine fractions (A-I). The Ethyl acetate extract and the all fractions were tested for antioxidant activities using radical DPPH (2,2-diphenyl-1-picrylhydrazyl) and antidiabetic activity using baker’s yeast and rat intestinal acetone powder. The inhibititon value (IC50) of fraction H was 0.03 µg/mL and fraction I was 0.06 µg/mL. Antidiabetic inhibititon value (IC50) using yeast of fraction H was 102.33 µg/mL and fraction I was 29.51 µg/mL, on the other hand antidiabetic inhibititon value (IC50) using rat intestinal of fraction H was 43.65 µg/mL and fraction I was 75.86 µg/mL. Based on monitoring data, it shown that the polar fraction H and I has the best inhibition activity than the other positive fractions, so that the active compound in fraction H and I need to be produced