This thesis contains four essays on non-parametric estimators of the spot volatility, the leverage and the volatility-of-volatility. In particular, the focus of this thesis is on the study of the asymptotic properties of the estimators, the optimization of their finite-sample performance and the use of the resulting estimates in empirical applications. Specifically, in Chapter 2 we prove a central limit theorem for the estimator of the integrated leverage based on the Fourier method of Malliavin and Mancino (2009), showing that it reaches the optimal rate of convergence and a smaller variance with respect to different estimators based on a pre-estimation of the instantaneous volatility. Then, we exploit the availability of efficien...