Characterization of multi-trait plant growth promoting Pseudomonas aeruginosa from chickpea (Cicer arietinum) rhizosphere

In the current perusal, 12 isolates of Pseudomonas were segregated by rhizospheric soil of chickpea (Cicer arietinum) of Madhya Pradesh, India. Isolated test organisms were characterized morphologically, biochemically and 16S rRNA gene sequencing. Out of 12, one isolate designated as P4 was identified as Pseudomonas aeruginosa through 16S rRNA gene sequencing, which revealed 100% homology with the strains DSM 50071 and NBRC 12689. The phylogenetic examination was accomplished utilizing MEGA-X to confirm the identity of isolate P4. The nucleotide hierarchy of the 16S rRNA gene from P4 isolate was submitted in the National Center for Biotechnology Information (NCBI) database under gene bank with accession number MT116414. The P4 isolate exhibited multiple plant growth promotion properties like phosphate solubility, indole acetic acid (IAA) production, 1-aminocyclopropane-1-carboxylate (ACC) deaminase, siderophores, ammonia (NH3) and hydrogen cyanide (HCN) activities, and biocontrol activities against phytopathogenic fungi Fusarium oxysporum and Macrophomina phaseolina