Maintenance of prospective cabbage (Brassica oleracea var. capitata) lines by micropropagation

With the aim to improve the breeding process of cabbage (Brassica oleracea var. capitata) and to develop new cultivars in a shorter period of time, as well as to develop a basis for use of biotechnology methods, the in vitro propagation of Brassicas, was investigated. The experiments included 39 genotypes of different Brassicas, most of them cabbages. Shoot culture was obtained from segments of aseptically germinated seeds and from lateral shoots from plants grown in the open field and under cover. Shoot culture media for optimal growth and multiplication were elaborated. "Microplants" were planted in the open field. All the important conditions for the cloning process were evaluated