TOTAL FLAVONOIDS CONTENT IN ACIDIFIED EXTRACT OF FLOWERS AND LEAVES OF GARDENIA (GARDENIA JASMINOIDES ELLIS)

ABSTRACTBackground: Gardenia (Gardenia jasminoides Ellis) contains secondary metabolites which responsible for pharmacological activities such as flavonoids. Objective: The aims of this study were to determine the total flavonoids content (TFC) in the flowers and leaves of gardenia (G. jasminoides Ellis)which macerated with acidified ethanol.Method: The method was the colorimetric of a colored complex between flavonoid with aluminum chloride.Results: The results showed that the highest TFC in the flowers (0.078% w/w) was obtained in a mixture solvent of ethanol and acetic acid, whereasin the leaves (0.090% w/w) was obtained in ethanol.Conclusion: There is no correlation between acidified ethanol and TFC in gardenia flowers and leaves.Keywords: Maceration, Acidified ethanol, Colorimetric, Aluminum chloride