TO EVALUATE HEPATOPROTECTIVE ACTIVITY OF LEAVES OF OCIMUM SANCTUM USING ANIMAL MODEL

ABSTRACTObjective: To evaluate the hepatoprotective activity of leaves of Ocimum sanctum in carbon tetrachloride (CCl4) induced hepatotoxicity in albinorabbits.Methods: This study was conducted on 12 healthy albino rabbits of either sex weighing 1.5-2.0 kg, divided into 2 groups. Hepatotoxicity was inducedin rabbits by CCl4 0.05 mg/kg, intra-peritonealy. Alcoholic extracts of leaves of O. sanctum was administered orally for 20 days from 1 day to day 20 inthe doses of 100 mg/kg/day with the help of a syringe.Results: Group I: The rise of serum transaminase (p<0.001), serum alkaline phosphatase (p<0.001), serum bilirubin (p<0.001) and decrease inserum albumin (p<0.001) due to hepatotoxic effect of CCl4 when compared to zero-day of same group (p<0.001). Group II: O. sanctum extract was ableto bring down the level of serum transaminase, serum alkaline phosphatase, serum bilirubin and increased in serum albumin in a statistical highlysignificant amount (p<0.001), when compared with Group I.Conclusion: O. sanctum leaves found to be effective in reducing serum glutamic oxalacetic transaminase, serum glutamic pyruvic transaminasealkaline phosphatase, serum bilirubin. O. sanctum extract helps in normalize serum albumin level. O. sanctum extract had shown protection in therestoration of liver function and regeneration of liver cells as observed on histopathology