IN VITRO ANTIBACTERIAL ACTIVITY AND PHYTOCHEMICAL ANALYSIS OF ORMOCARPUM COCHINCHINENSE EXTRACTS ON NOSOCOMIAL INFECTION CAUSING BACTERIA

Objective: The present study was aimed at evaluating the antibacterial activity and phytochemical analysis of the leaf extracts of Ormocarpumcochinchinense.Methods: The leaf powder was extracted by sequential extraction method using chloroform, acetone, and methanol. Preliminary phytochemicalanalysis of the extracts was done using standard methods. The dried extracts were screened for antibacterial activity using disk diffusion method andthe minimum inhibitory concentration (MIC) method.Results: The acetone extract showed the presence of flavonoids, tannins, steroids, phlobatannins, and saponins. Methanol extract containedflavonoids, tannins, phlobatannins, saponins, and terpenoids. The chloroform extract contained steroids. The chloroform extract did not show anyactivity, whereas the acetone extract showed significant activity against all the bacteria with a MIC value of 0.080 mg/ml against Escherichia coli(ATCC 25922) and Klebsiella pneumoniae (ATCC 15380) and 0.18 mg/ml against Staphylococcus aureus (meticillin resistant). The methanol extractshowed very less activity.Conclusion: The present study has proved the antibacterial potency of O. cochinchinense for the first time. This plant is a potential source ofantimicrobial agents of the future.Keywords: Antibacterial activity, Phytochemical analysis, Minimum inhibitory concentration, Ormocarpum cochinchinense, Acetone extract