ANTIBACTERIAL EFFECT OF LANTANA CAMARA LINN. ON GRAM NEGATIVE BACTERIA AND NDM-1 STRAINS: AN INVITRO STUDY

Abstract:Aim:  To evaluate the antibacterial effect of lantana camara Linn. on gram negative bacteria and NDM strain by in vitro method .Methodology: The antibacterial activities of leaf extract (LELC) of lantana camara alone or in combination with Gentamicin,Ceftrioxone were determined by in vitro study such as disc diffusion and agar dilution methods. Fully characterized, stocked strains of Gram negative bacteria of Escherichia coli (ATCC25922) , staphylococcus aureus ( ATCC 25923) and fully characterized known NDM-1 strains of Klebsiella pneumonia were used for the study.Results: The results showed the inhibitory activity of LELC against E.Coli (3.5mm ),S.aureus (4 mm), and NDM1 Strains( 1.2mm). The synergestic effect of the extract and gentamicin (5.5 mm)against E.coli , extract and ceftrioxone (6 mm)against S.aureus. Notable effect was obtained with LELC with gentamicin against NDM 1 strain with zone of  inhibition  (2.2 mm). In the agar dilution method MIC of LELC against E.Coli ( 500mcg),S.aureus (500mcg ), but the combination of LELC and gentamicin only showed MIC of(500mcg+100mcg ) against NDM1 .Conclusion: The LELC shows antibacterial activity against gram negative bacteria especially the MDM1 producing bacteria which may be developed as a future antimicrobial agent against resistant gram negative organisms.Key words: Lantana camara, antibacterial effect, gram negative bacteria, NDM1 strains  Â