ANTIBACTERIAL ACTIVITY OF VULGAROL A EXTRACTED FROM THE LEAVES OF SYZYGIUM CUMINI

Objectives: Extraction, purification and identification of antimicrobial compounds from leaves of Syzygium cumini.  Methods: The antibacterial activity of crude extract of Syzygium cumini leaves were investigated against pathogenic Gram positive and Gram negative bacteria. Petroleum ether extract was used for the separation of compound using thin layer chromatography and separated compound identified by using Gas Chromatography-Mass Spectroscopy.Results:Extracts prepared in various organic solvents such as chloroform, methanol, petroleum ether, acetone and ethanol showing antibacterial activity against Salmonella typhimurium, Pseudomonas aeruginosa and Staphylococcus aureus, but significant activity found with petroleum ether extract. The purified compound from petroleum ether extract showing antibacterial activity was identified as diterpenoids i.e. Vulgarol A by Gas Chromatography-Mass Spectroscopy. Vulgarol A shows more potential antibacterial activity against Gram negative organisms such as P. aeruginosa.Conclusion:This study concludes that the extracted compound Vulgarol A from Syzygium cumini leaves could be used as an active pharmaceutical ingredient to control infectious diseases caused by P. aeruginosa. Keywords: Syzygium cumini, diterpenoids, P. aeruginosa, GC-M